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Bacterial Concentration and Diversity within Repetitive Aliquots Collected from Replicate Continuous-Flow Bioreactor Cultures
Tawni L Crippen, *
, 1
Cynthia L Sheffield1
Kathleen Andrews1
Roy Bongaerts2
David J Nisbet1
Authors Info & Affiliations
, 1
Cynthia L Sheffield1
Kathleen Andrews1
Roy Bongaerts2
David J Nisbet1
Authors Info & Affiliations
Abstract
The aim of this study was to determine the reproducibility of small volume repeat sampling from replicate bioreactors with stabilized continuous-flow chicken cecal bacterial communities. Bacterial concentration and diversity were analyzed by phenotypic, biochemical and ribotype analysis. Significant differences in concentrations and variations in diversity were found in replicate bioreactors.
Keywords: Bioreactor, cecal, chicken, continuous-flow culture, repetitive sampling.